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Investigators recognized mechanistic inconsistencies implied by the structural data, and a decade of research finally yielded a structure corresponding to a reactive state.
Guided by the structural data, we engineered a SpCas9 variant that specifically recognizes NAAG PAMs.
This suggests that generation of the single-stranded attC by the enzyme acts via a different way, as suggested by the structural data [15], probably by strong interaction between IntI1 and the extrahelical bases in the recombination site, leading to opening of the strands.
As predicted by the structural data, site 2 mutations had a more significant effect on signaling in the Wnt reporter assay.
Chemical modifications of this compound (i.e. of the C5/C6-carbonyl groups) can be explored with the help of the binding mode revealed by the structural data presented here.
Although these data emphasise the importance of this residue in overall peptide binding, it is clearly impossible to discern any contribution of aprataxin Arg42 to the additional affinity accrued from accessory serine phosphorylation implied by the structural data.
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For modelling a complicated 3-D vehicle cabin model, the structural acoustic coupling system is analyzed by combining the structural data from modal testing with the acoustic data from the finite element method.
By using the structural data of the single crystals of the complex, Rietveld refinements were carried out with the program TOPAS by using the fundamental parameter approach.
The resulting set of variance images can either be thresholded manually or by using the structural data, which is typically more effective in the presence of residual motion [ 18].
In part, these ambiguities were caused by indistinctness in interpretation of the structural data used.
In the second analysis we attempted to partial out the variance in the structural data accounted for by generic cognitive factors by including five further regression variables.
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