Thus, RNAP II degradation can be monitored by the change in the staining pattern seen with H5 antibody [11], [36], [37].
Slides were reviewed independently by two observers to evaluate the staining pattern of the protein separately under the light microscopy.
This preferential detection of pathological TDP-43 inclusions with minimal or no normal nuclear TDP-43 staining by IHC is similar to the staining pattern seen with pathology-specific phospho-TDP-43 specific MAbs such as the p409/410 MAb mentioned above.
As current MS sensitivity is at the low femtomole to the upper attomole level, it surpasses the detection limit of silver stained gel, leading to the preferred analysis to cut whole gel lanes into pieces, irrespective of the staining pattern, followed by trypsin digestion and analysis by LC MS MS.
The epithelial region was segmented using the staining pattern produced by both pan-cytokeratin and E-cadherin antibodies.
In prostate cancer cells stimulated with TNF- α, the antibody exhibited strong nuclear staining, as assayed by immunofluorescence, while in nonstimulated cells the staining pattern was mainly cytoplasmic.
The staining pattern exhibited by these purified antibodies is consistent with the localization of PC1 in the Golgi, as evidenced by its colocalization with p115 and secretory granules of the RSP.
In intact spreading cells, the staining pattern made by an antibody against the extended p130Cas substrate domain shows a similar staining pattern to that made by an antibody against phosphorylated p130Cas in the cell periphery, 34 suggesting that the p130Cas substrate domain is extended by force, and tyrosine-phosphorylated by p60Src.
Importantly, in fixed liver sections of P. berghei-infected mice, the staining pattern obtained by anti-PbSERA3-N antiserum detection resembled the in vitro acquired results with PbSERA3-N localizing mainly in the parasite cytoplasm and close to the PVM (Fig. 5B and C).
