Sentence examples for by the stable expression from inspiring English sources

To assure that the RNAi pathway was activated by the stable expression of shRNAs and did not interfere with HIV-1 replication leading to off-target effects, we used scrambled shRNA (shSCRAM) as control.

Targeted lines were selected by mobilizing and linearizing the original donor using hsFLP and hsI-SceI and crossing these virgin to eyFLP [40] males so that reintegration can be detected in the progeny by the stable expression of the white+ reporter.

Fig. 6A shows that, with mTORC1, the change of activity induced by the stable expression of p75NTR was minor.

Moreover, HSF1 was not activated as reflected by the stable expression of HSP70, the protein that is most highly responsive to proteotoxic stress.

Hypoxia-inducible factor-1 α function was suppressed by the stable expression of a dominant-negative form in HCT116 cells (DN).

The role of mitochondria was examined by the stable expression of Bcl2 or stable knockdown of SMAC in combination with up- or downregulation of XIAP expression.

Our analyses identify three general ways in which parental effects can change the adaptive value of an adult behavioural trait: by influencing the associated fitness benefits and costs; by consequently changing the evolutionary outcome of social interactions; and by modifying the evolutionarily stable expression of behavioural traits that are themselves parental effects.

As Brca1 is essential for cell survival, we immortalized the Brca1 F/− MEFs by stable expression of the p53 shRNA or SV40 large T antigen (LT), a suppressor of both pRb- and p53-dependent senescence pathways.

Therefore, it appears that the majority of the phenotypic changes, induced by ICN stable expression, are achieved by restoring endogenous TGF-β signaling, repressed as a consequence of v-Src activity.

The W76X mutation prevents the encoded p27 protein from entering the nucleus, the cell compartment where p27 exerts its function as a cell cycle inhibitor, and indeed p27W76X can no longer inhibit the growth of GH3 cells both by clonogenic assay and by stable expression.

Gregory et al. [ 47] induced EMT in MDCK cells (human Madin Darby canine kidney epithelial cells) either by transforming growth factor (TGF -β or by sTGF -βexpressior of the tyrosine phosphatase Pez.