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Image acquisition was controlled by the software LaserSharp2000 (Biorad).
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The hysteresis curve was directly produced by the software (Datamanager 96, Dartec Ltd ,Stourbridge, UK) in our experimental setup from a continuous flexion-extension movement.
plecostomus brain EST database was performed using the software blast-2.2.19 blast-2.2.19 NCBI.
The July 2007 Mus musculus genome assembly (NCBI37/mm9) from NCBI was used for the sequence alignment by the software Bowtie (0.12.7) (Langmead, 2010).
The minimum free energy calculated by the software was -44.70, -38.84 and -30.70 kcal/mol for the donor splice sites downstream of the exons c, c1 and 1b, respectively.
Statistical comparisons of the AUC for each parameter were performed with the DeLong test [ 17] by the MedCalc software 12.7.0 trial version (MedCalc Software bvba, Belgium).
Due to the decrease in overall signal intensity, less spots were flagged as 'present' by the quantification software (3740 ± 322 at 16 h, compared to 4820 ± 172 at 4 h), but we show here the intensity of all spots.
The July 2007 Mus musculus genome assembly (NCBI37/mm9) from NCBI was used for the sequence alignment by the software Bowtie (0.12.7) [ 53].
After eliminating positions with gaps, the gene genealogies were assessed by maximum likelihood using the software package PAUP*4.0b10 (Swofford 2003).
Hydrochemical results of all samples were statistically analyzed by the software STATISTICA® (1998).
Coding was supported by the software MAXqda 2001 (VERBI GmbH Berlin).
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