In a protocol approved by the Animal Care and Use Committee of the University of Pennsylvania, alveolar type II cells were isolated from male Sprague Dawley rats based on a method reported by Dobbs et al. [ 34], with the slight modification reported earlier [ 35].
The vectors pHFim1eGFP, pHeGFPRab5 and pHeGFPRab7 were transformed into A. tumefaciens strain EHA105 by heat shock method (Holsters et al., 1978) and A. tumefaciens mediated transformation of Z. tritici was performed as described previously by (Zwiers and De Waard, 2001) with the slight modifications.
The transition from maintenance to destruction of the network is very sharp leading to the conclusion that the network's fate might change by a slight modification of the parameter controlling the transition - almost like a phase transition.
The tube was correctly positioned in relation to the carina by using a slight modification of the method described by Evron et al. 14 Specifically, the bronchoscope was advanced to the distance of the carina.
Subsequently, an appropriate-sized connective tissue graft was removed from the palatal mucosa between the first molar and the canine by using a slight modification of the single incision technique [ 10, 16].
Our first generation Tet-regulated, prostate-specific (PS/TR) vector [ 9] was developed by a slight modification of the Tet-off system in which the tTA was driven by the prostate-specific ARR2PB promoter instead of by the CMV promoter (see Fig. 1).
By a slight modification, we use the methods and the framework of [3, 5] to prove Theorem 2.2.
Compounds were prepared either using the conditions described above or by slight modification of the procedure.
By exactly slight modification to the proof above, we can prove (ii) if l = + ∞. □.
The zero eigenvalues can be shifted to any prescribed value and thus removed by a slight modification of the second approach.
