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Split-CreERT2 allows controlling DNA recombination spatially by the simultaneous activity of two promoters and temporally by application of tamoxifen.
We have developed a conditional and inducible DNA recombination system for cell populations defined by the simultaneous activity of two promoters.
In this way, split-CreERT2 will provide a new tool of modern genetics allowing spatial and temporal precise genetic access to cell populations defined by the simultaneous activity of two promoters.
A lower number of proteins in the bilayer however can be beneficial for the resolution of single channel activity because individual events are not masked by the simultaneous activity of many channels.
We present here a powerful tool which allows selective genetic targeting of cell populations defined by the simultaneous activity of two promoters in the mouse, thereby adding a second dimension of cell-type specific control to Cre-based conditional mouse genetics.
Placing both split-Cre proteins under the control of two different promoters, we observed DNA recombination in the brain of living transgenic mice in a cell population, which was defined by the simultaneous activity of the human glial fibrillary acidic protein promoter as well as the mouse proteolipid protein promoter [15].
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The spatial aspect is covered by including the simultaneous activity of multiple neurons.
Next, temporal relation of the simultaneous activity patterns set is determined between the sets by using the Allen's temporal relation.
The simultaneous activity patterns are constructed as the simultaneous activity patterns set[20].
In the simultaneous activity pattern set, the activity has the probability relationship with another activity, and the simultaneous activity pattern set has the probability relationship with another set.
However, ISM permits an activity to be included in multiple sets in the process of constructing the simultaneous activity patterns set, that is, confusion of service control can occur by service activity duplication.
More suggestions(16)
by the simultaneous production
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