Sentence examples for by the shotgun method from inspiring English sources

In this study, a DNA fragment including the open reading frame of mpd (methyl parathion hydrolase encoding gene) and cognate regulator of a methyl parathion (MP -degrading strain PseudoMP -degradingDLL-1 wastrained by the shotgun method.

A single full plate run of 454 Titanium (Roche Applied Science) was performed per library using 3 µg of normalized cDNAs processed by the "shotgun" method.

The DNA of CojaIIB, Tapasin and BRD2 positive cosmids was sequenced by the shotgun method [ 46].

High-quality BAC sequences were determined by the shotgun method using the Sanger sequencing protocol, as described previously.

The genomic sequences derived from 311CIIB-18, 311CIIB-20 and 322CIIB-02 cosmids were determined by the shotgun method.

The nucleotide sequences of the BAC clones were determined by the shotgun method using an automated sequencer (Applied Biosystems, model 3700).

We subjected the PaP1 genome to re-sequencing in another sequencing company by using the shotgun method.

The nucleotide sequence of the pEP5289 plasmid of Neisseria gonorrhoeae clinical isolate 5289 was determined via the shotgun method by Macrogen Inc. (Seoul), resulting in 7 contigs with at least 6 times coverage.

But in a series of e-mail messages from February onward, and in a public lecture at Harvard last month, Eric Lander, one of the three principal members of the consortium, has derided the shotgun method championed by J. Craig Venter, Celera's president.

A DNA fragment library of PhcyNPV was constructed using the shotgun method described by Zhu et al. [ 31].

By contrast, the PaP3 genomic DNA [ 19], successfully sequenced using the shotgun method, cannot be degraded by Endo V under the same reaction condition; this result suggested that no Endo V cutting site exists in the PaP3 genome.