Sentence examples for by the shRNA vector from inspiring English sources

Knockdown of a specific gene by the shRNA vector can lead to three possible cellular responses to this treatment.

The HOTAIR knockdown reduced cancer cell metastasis in vivo, and the tumours formed by HOTAIR-silenced ESCC cells were smaller, both in size and weight, than the tumours and metastases formed by the shRNA vector control cells in a mouse xenograft model.

In addition, we tested for both ARNTL and TNIP1 if protein levels were also affected by the shRNA vectors.

The efficiency of target silencing was then analyzed by transiently transfecting the shRNA vector in Cos cells with two vectors expressing firefly luciferase and Renilla luciferase, and luciferase activity was analyzed 48 h and 60 h after transfection.

The activity of the L6 promoter is dramatically reduced by the cotransfection of the shRNA vector in both MCF-7 and T-47D cell lines, indicating that GABP alpha/beta is an important regulator of the BRCA1 promoter in these lines.

The shRNA vector was synthesised by Invitrogen using the oligonucleotides and pENTR/H1/TO (Invitrogen).

The enhancement in Bim levels and apoptosis induced by PTK6 shRNA expression can be fully suppressed by co-expression of wild-type PTK6 that cannot be targeted by the PTK6 shRNA vector, supporting the specificity of this regulation (Fig.  4b).

The tumours formed by the HOTAIR-silenced cells were smaller both in size and weight than the tumours formed by the cells expressing the control shRNA vector.

However, tumours formed by TBLR1-silenced cells were smaller, in both size and weight, than the tumours formed by shRNA vector cells.

In contrast, the expression of the shRNA through intron splicing was completely abolished in 293T cells, although the silencing induced by the shRNA-expressing vector alone was no different from that in the Cos cells.

KRT23 expression was successfully reduced by both shRNA vector constructs; albeit to different levels depending on the KRT23 sequence targeted by the two different shRNA vector constructs.