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The pathway converts pyruvate to leucine by the sequential reactions described in Figure 14.
(A ) Schematic depiction of the tetrasaccharide structure produced by the sequential reactions of B4GAT1dTM followed by LARGEdTM with the sugar units labeled A-D to indicate the order of their addition.
In plants, glutamate is converted to proline in the cytosol and potentially in plastids by the sequential reactions of pyrroline-5-carboxylate (P5C) synthetase (P5CS) and P5C reductase (P5CR) [ 1].
Saturated, unbranched very long-chain fatty acids (VLCFA; fatty acyl-chain length of ≥22 carbons) are degraded in the peroxisomal matrix by the sequential reactions of the enzymes (acyl-CoA oxidase 1, D-bifunctional protein and either peroxisomal β-ketothiolase 1 or sterol carrier protein x) of the β-oxidation pathway.
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Cysteine biosynthesis, achieved by the sequential reaction of two enzymes, serine acetyltransferase and O-acetylserine (thiol) lyase (OASTL), represents the final step of sulfur assimilation pathway in plants and bacteria.
Besides regulation by transcription factors, modulation can be achieved by compounds formed along the sequential reactions of interconnecting pathways that accumulate and bind directly to the allosteric sites of the cognate enzymes.
The sequential reactions catalyzed by the elongase, STA → ETA and EPA → DPA in the n-3 pathway and GLA → DGLA and ARA → DTA in the n-6 pathway, provide an abundance of precursor fatty acids for EPA and ARA synthesis (in the first reaction) and a progenitor of DHA (in the second reaction).
Beads were deposited on slides upon which the sequential reactions and washing steps were done automatically by the SOLiD™ 3 instrument.
Moreover, the synergy degree of the simultaneous reactions was higher than that of the sequential reactions.
PEMT activity in aged CC increased by 100%, the principal modification being observed in the first methylated product of the sequential reaction.
The reaction characteristics on the metallosilicate catalysts were explained by the microscopic sequential reaction (MSR) mechanism.
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