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One explanation for this could be tumor heterogeneity, which would render mutations present in small subset of cells undetectable by the sequencing technology used.
Apart from the aforementioned problems, the analysis of metagenomic data sets can also be influenced by the sequencing technology used.
This specifies relative abundance of each gene variation between RNA samples and also measurement error estimated by the sequencing technology.
To exclude false positives introduced by the sequencing technology or the mapping algorithm used, results were confirmed with Sanger sequencing.
Also we demonstrated in the Pacific Biosciences experiments that the choice of assembly method should be informed by the sequencing technology and desired result.
Technical replicates from the same library are typically not necessary, due to the high technical reproducibility achieved by the sequencing technology [ 17].
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Although the procedure is conceptually simple, technical difficulties arise due to inhomogeneous samples, existence of competing subclonal populations, and systematic and non-systematic errors introduced by the sequencing technologies.
Based on these considerations we can assume that our results should not be affected too much by problems with the sequencing technology.
However, the mutation detection levels are limited by the error rate of the sequencing technology as well as by the availability of variant-calling algorithms with high statistical power and low false positive rates.
The low percentage (21.4%) of cucumber unmappable unigenes that can be assigned a putative function might be mainly due to the short sequence reads generated by the 454 sequencing technology and the relatively short sequences of the resulting unigenes (Table 1 and 2), most of which probably lack the conserved functional domains.
comp53892_c1 annotated as encoding aldehyde dehydrogenase can not be detected in the shoot due to no/low expression, so as the result by the Illumina sequencing technology.
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