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16S rDNA sequencing confirmed the bacterial identification and allowed us to detect differences between two sequences of the same H. kunzii type strain (gene Bank accession numbers KM403387 and X69837) which could be explained by the sequencing methods performed and will have to be investigated by whole sequence analysis.
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Results and conclusions: Among the total of 110 consecutively collected serum specimens submitted for HCV genotyping, 108/110 could be typed by the sequencing method and 107/110 were typable by LiPA HCV II method.
New methods for analyzing pyrosequencing output suggest that much of the rare microbiome is composed of errors introduced by the sequencing method [10].
In order to estimate the error introduced by the sequencing method, the same procedure was applied to the PhiX174 control lane of the sequencing run.
One strain identified as Mycobacterium genus by GenoType was not recognized by MALDI-TOF and was identified as M. duvalii by the sequencing method at the Mycobacterium Reference Laboratory.
With the exception of nucleotide qualities, which are provided as input by the sequencing method, all log-likelihoods are sampled from the data itself.
To reduce false positive variant calls introduced by the sequencing method, we applied specific trimming, filtering and variant calling parameters in the CLC Genomics Workbench software.
A close relationship between HRT and BRS, as assessed by the sequence method, was found by Lin et al. [ 16] in 16 patients without structural heart disease, who were studied before and after sequential sympathetic, parasympathetic and combined autonomic blockade.
Throughout this chapter we will describe the main computational challenges imposed by the shotgun sequencing method, and survey the most widely used assembly algorithms.
The sequences produced by random amplification and RPM version 1 were identical to those identified by the conventional sequencing method with the exception of ambiguous base calls (Ns).
The genomic DNA PCR product from each individual plant was directly sequenced by the Sanger sequencing method and the sequence chromatograms visually inspected for sites where two nucleotide peaks of similar height were present which indicated heterozygosity.
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CEO of Professional Science Editing for Scientists @ prosciediting.com