Sentence examples for by the sequencing coverage from inspiring English sources

The ratio of transitions to transversions was approximately 1.77 and likely caused by the sequencing coverage and filtering steps.

After normalizing by the sequencing coverage, viral reads represented at most 0.0004‰ of all the reads in our samples, compared to 0.1‰ in the positive control.

In processing the raw single nucleotide polymorphism (SNP) data, we used a threshold of 40 on the Phred score and normalized the allele frequency by the sequencing coverage, following Liti et al. (2009).

However, we expect that a small number of bona fide but low abundance Tribolium miRNAs may be missed by our strategy, where the miR* sequence falls below the detectable limit imposed by the sequencing coverage.

Additionally, we detected a high degree of aneuploidy (3.6%) by examining the sequencing coverage in these segregants.

This bias is independent of the sequencing platform and cannot be avoided nor removed by increasing the sequencing coverage or the length of the reads.

The 454 pyrosequencing single-end shotgun data presented difficulties that would, in several cases, not have been ameliorated by increasing the sequencing coverage (Additional file 1: Figure S1D).

The results demonstrated that a mixing ratio of two DNA sources as low as 1 250 can be detected, and the authors concluded that by increasing the sequencing coverage, a mixing ratio of 1 1000 might be detectable as well [26].

With the knowledge that there are 6 Itln genes in the 129S7 mouse and that it has a similar Southern pattern to the other 129 substrains, an attempt to identify the Itln CNVs in these strains was done by comparing the sequencing coverage across the Itln locus.

We evaluated the sequencing coverage by examining the number of 16S rRNA gene sequences generated per swab sample, which showed that the lowest number of sequences per sample to be n = 387.

The taxonomic and functional profiles were normalized to determine the differences in the sequencing coverage by calculating the percent distribution prior to downstream statistical analysis.