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ZFRs, like ZFNs, have the potential to target many new sequences, being limited in principle only by the range of sequences for which specific zinc finger domains can be made and by the sequence selectivity of the catalytic domain [1].
Localization is compromised by the sequence selectivity of RNase digestion.
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In each case the sequence selectivity was confirmed by significant lowering of the melting temperature.
The results reconcile previous conflicting experimental models for the sequence selectivity of the recognition process, by confirming many of the findings while identifying important new interactions and behaviour.
Nonetheless, the sequence selectivity rules are useful in analyzing the experimental results.
Here, we define the unbiased sequence selectivity of the tru pathway with the heptapeptide series.
Here, we examined the absolute sequence selectivity of the trunkamide cyanobactin pathway, tru.
By using tandem mutations, we were able to determine sequence selectivity regardless of the context of the amino acid.
Related derivatives were also evaluated for sequence selectivity on the same DNA sequence to establish structure binding selectivity relationships.
The observed "side-by-side" binding of small polypyrrole antibiotics has led to the design of synthetic hairpin polyamides with programmable DNA sequence selectivity.
Additional residues within the basic region confer further DNA binding site sequence selectivity.
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