Your English writing platform
Discover LudwigExact(1)
Some property differences of the isomers caused by the sequence changes were found.
Similar(59)
s/m indicates whether (s)ingle or (m)ultiple nucleotides were affected by the sequence change.
Finally, alternative splicing events belong to the third class when both the catalytic and the binding domains are affected by the sequence change.
With a long-term objective of developing a community resource to quickly identify mutants for a target gene; here we report an efficient approach to identify induced mutations by accurately differentiating those from the sequence changes present among the three wheat homoeologs.
MSP distinguishes unmethylated from methylated alleles based on the sequence changes created by bisulfite treatment of DNA, which are recognized by PCR with one of two pairs of primers designated for either methylated or unmethylated DNA.
The sequence changes detected by Illumina resequencing of these strains are shown in File S1.
Each of these approaches requires significant manual examination of the results to extract true-positives from a high background rate of spurious predictions and to precisely describe the sequence changes caused by mutations that lead to structural variation.
Some of the sequence changes were then validated by the dye-terminator method.
A great deal of information on the functionality of a sequence can be learned by studying how the sequence changed throughout evolution.
Supplementary Figure 5 Restriction enzyme digestion showed TAPS effectively converts 5mC to T. (a) Illustration of restriction enzyme digestion assay to confirm the sequence change caused by TAPS.
This analysis identified 22 sequence variants colocalizing with shared/conserved ESEs located within 125 nucleotides of exon junctions (Table 3), with a total of 14/23 (61%) of the predicted ESEs affected by these sequence changes (Table 4).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com