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Phylogenetic compositions of bacteria in anode biofilms, cathode biofilms, and electrolyte suspensions in MFC-W and MFC-M were determined by the sequence analyses of amplified 16S rRNA gene fragments (Fig. 4).
Two isolates lacked cfp32 PCR product and one lacked RD12, however, those three samples were further examined and identified as M. tuberculosis by the sequence analyses of hsp65 and gyrB.
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Some strains were differentiated by the PFGE patterns of SpeI digests and by sequence analyses of the lsc and the ppiD genes, and the Chinese Gibbsiella strain was most divergent.
Whereas the CCMV CP construct containing the C-terminal 6xHis-tag became well expressed (Fig. 3b1), and the presence of the 6xHis-tag encoding nucleotide sequence was verified by nucleotide sequence analyses, the His-tag could not be detected on western immunoblots using anti-His serum (Fig. 3b2).
While this normalization may be expected to reduce the representation of differentially expressed genes in male and female, the deep sequence analyses provided by the 454 sequencing platform were able to produce data exhibiting some gender specific gene transcripts.
The combination of the rapid identification of natural null mutations by exome sequence analyses along with the option to establish brother-sister mating in a multiparous animal will facilitate the development of models based on homozygous natural null mutations.
Some of the positive samples were further characterized by sequence analyses of the HSP70 gene (the two from pet shops and another two from kennels) and actin gene (the two from pet hospitals, the two from pet shops, and another four from kennels).
The vosA ORF was determined by RT-PCR followed by sequence analyses.
All four substitutions were confirmed by nucleotide sequence analyses of the constructed vectors.
Successful incorporation of the mutations was confirmed by DNA sequence analyses, using forward and reverse primers to check the entire AQP1 sequence, and confirm the absence of random mutations.
By sequence analyses, the majority were found to carry mutations in both SlTRM3/4 and SlTRM5 and occasionally mutations were in both alleles of one or both genes.
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