Sentence examples for by the sequence accuracy from inspiring English sources
We explain the difference from previously published estimates of 25 and 21 mya [ 3, 5] by the sequence accuracy of finished versus whole-genome shotgun sequences [ 6].
The sequence accuracy was confirmed by two-directional sequencing and by sequencing a new PCR product if necessary.
While related approaches increase the sequencing accuracy by combining multiple MS spectra from overlapping peptides (Bandeira et al., 2004; Bandeira et al., 2007a; Shevchenko et al., 1997), the experimental setup described here requires fewer sample handling steps (no need to digest with multiple enzymes) and should thus be applicable to smaller amounts of substrate.
The sequencing accuracy was increased by using overlapping paired-end reads as illustrated in Figure 3 figure supplement 1.
The error rate could be further reduced by selecting the oligonucleotides with the highest sequence accuracy identified in a comparative test of three suppliers (Table 1).
Apollo software was used to confirm the protein sequence accuracy by transcribing, and translating the DNA, identifying untranslated regions (UTR), translation start, exons, introns, and translation stops.
The probe sequence accuracy was validated by mapping the sequences to the Ae. tauschii Illumina reads representing 30X genome coverage.
The estimated sequence accuracy of the total repeat region was 99.8% based on a comparison with the reference sequence.
The high level of sequence accuracy is further demonstrated by the identification of known polymorphisms.
The DNA barcoding movement made an important impact on sequence accuracy by promoting a clear set of standards for DNA barcodes: raw sequence reads and reliable sequence data combined with a correct taxonomic name as well as collection and voucher information (26, 27).
For Roche/454 sequencing, indel errors are known to increase with increasing homopolymer length, while there is evidence to show that Illumina sequencing accuracy can be influenced by the sequence up to 10 bases before the current position [ 9].
