Sentence examples for by the second strand from inspiring English sources

The first-strand cDNA synthesis was followed by the second strand synthesis.

This suggests that second strand priming proceeds efficiently and that the first strand cDNA is successfully converted to double stranded cDNA by the second strand synthesis reaction.

Twenty micrograms of total RNA was used initially for the first strand synthesis, followed by the second strand synthesis using the SMART™ PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA) following the manufacturer's instruction.

Twenty micrograms of total RNA was used initially for the first strand synthesis, followed by the second strand synthesis using the SMART™ PCR cDNA Synthesis Kit (Clontech, BD) following the manufacturer's instruction.

First strand cDNA synthesis by random hexamers and reverse transcriptase was followed by the second strand cDNA synthesis performed using RNAseH and DNA Pol I. Double stranded cDNA was end repaired, 3´adenylated and the 3´-"T" nucleotide at the Illumina adaptor was used for the adaptor ligation.

Strand specificity is achieved by quenching the second strand during final amplification thanks to incorporation of dUTP instead of dTTP during second strand synthesis.

The RNA templates were then removed by RNaseH and the second strand was synthesized using DNA polymerase I to generate double-stranded cDNA fragments.

The first strand of cDNA was synthesised using random hexamer primers, followed by synthesis of the second strand.

First strand cDNA was synthesized using the binding mRNA as a template, and followed by the synthesis of the second strand of cDNA.

The second cDNA strand was synthesized by the addition of the second strand master mix and incubated at 16°C for 2 hrs.

Reverse transcription was performed on captured RNAs followed by the synthesis of the second strand of cDNA.