Sentence examples for by the second passage from inspiring English sources
We found that the two ER+ samples that successfully grew propagatable tumors in SCID mice lost their ER expression generally by the second passage (even when the mice were supplemented with estrogen).
The allantoic fluid of the inoculated embryos was positive by HA after the second passage of the virus in the SPF chicken embryos.
Spirochetes in the chipmunk's blood were passed through 4 laboratory mice and isolated in the second passage by using in vitro culture.
CFU-F were evaluated by culturing 2.5 × 10 cells at the second passage in 6-cm culture dishes.
The non-probabilistic reliability with consideration of time-dependency for servo mechanisms is obtained by the first passage theory and interval process model.
By the seventh passage, the vaccine strains could no longer infect nervous tissue or cause paralysis.
By the third passage 100% of cells were fibroblasts (Thy-1+, CD45-, CD68-).
By the third passage AR and PSA expression had decreased to 37% and 64% respectively of control cells transduced with virus vector alone and by the eighth passage expression of AR and PSA was almost non-detectable.
The upregulation of COMP gene expression back to primary fibrochondrocyte levels by the third passage was another exciting finding.
By the fourth passage, the cells are able to be cultured in the absence of BM stroma (Fig. 1C).
