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The length effect of MWCNTs evaluated by the second assay was detected earlier (at 24 h) by trypan blue counting than revealed by the CellTiter-Blue assay.
The serum leptin levels as assessed by two different immunoassays were very low in all three patients (1.6, 0.9, and 1.1 ng/ml by the first assay as well as 1.3, 1.2, and 1.1 ng/ml by the second assay, respectively) [15].
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The cheapest assay combinations are those involving the use of SD Bioline and First Response assays, with SD Bioline as the first assay followed by First Response as the second assay or vice versa.
VL values measured as <400/cc by the first assay were assigned a value of 200/cc and all the VL values were log-transformed prior to analysis.
The third assay used by Kotsopoulos et al (2007) was the micronucleus test (MNT) with some variation compared to the most frequently used protocols for the assessment of DNA repair capacity.
The first assay was realized by Hwang et al. (2004).
In addition, some chronically HCV infected persons may test HCV seronegative, particularly by the second generation assay mainly used here.
If this assay was positive, the result was confirmed by a second assay using a second set of primer pairs, 342F (5'-AGATGATGGATCTGCTTTCTTATCTG) and 492R (5'-AACCCTGTCCTGCACCCC), which targeted a different DNA sequence.
If this assay was positive, the result was confirmed by a second assay using a second set of primer pairs TW13F (5'-TGAGTGATGGTAGTCTGAGAGATATGT) and TW163R (5'-TCCATAACAAAGACAACAACCAATC) and a Taqman* probe (13F-163R, 6-FAM-AGAAGAAGATGTTACGGGTTG-TAMRA) targeting a different 150-bp sequence [ 20- 22].
At the population level, SLAG vision assessment can be validated by a second assay that is based on a different innate response, Dark Chamber preference.
and confirmed by a second assay with Uni-Gold (Trinity Biotech PK, Ireland), according to the national protocol at the time.
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