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Increased background of the spectrum is caused by the sample fluorescence.
The background of the sample labeled TiGO_075 is increased by the sample fluorescence.
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At 0 and 72 h of growth, samples were stained with Nile red and the lipid content of the culture was evaluated by measuring the sample fluorescence by fluorescence microscopy and flow cytometry.
Cell viability was quantified by measurement of the sample fluorescence intensity at 560EX/590EM.
Relative transcript abundances were determined by comparing sample fluorescence signals to dilution series of cDNA prepared from the 1 h WOS -treated samples, and examined on the same plate.
Permeability was calculated by linear regression of sample fluorescence (Excel 5.0, Microsoft Office).
Paracellular flux was calculated by linear regression of sample fluorescence (Excel 5.0, Microsoft WA, Power Macintosh 7200).
Freely emitting peptide fragments that were released by thrombin activity rapidly increased sample fluorescence (red, Figure 1 C).
Sample fluorescence was determined by averaging the three readings and subtracting the fluorescence of a ThT blank.
The fluorescence emitted by the sample was collected by the same objective lens and separated from the excitation light by a custom-designed dichroic mirror (AHF Analysentechnik, Tuebingen, Germany).
Fluorescence emitted by the sample was collected by the same objective lens and passed through a 532 LP dichroic mirror LPD01-532RU-25x36x1.1, Semrock, Rochester, NY), a 532 LP sharp edge filter (LP03-532RU-25, Semrock).
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