Sentence examples for by the same secondary from inspiring English sources
Nonspecific fluorescence was assessed by using an isotype-matched irrelevant mAb (R&D System) followed by the same secondary antibody.
For examination of apoptosis, sections were incubated with anti-cleaved caspase-3 (1 200; Sigma) and anti-BAX (1 50; Santa Cruz Biotechnology), followed by the same secondary antibodies.
Because our tRNA is reconstructed with a high score, and tRNA-Trp was inferred confidently in another location (see below), we consider the similarities with tRNA-Trp as a result of convergent mutations constrained by the same secondary structure.
Additional experiments were performed in the LPK animals with all three antibodies as a triple label, as γ-tubulin and α-tubulin antibodies identified discrete structural regions (basal body and ciliary shaft, respectively) and could be distinguished by location, despite both being identified by the same secondary anti-mouse Dylight 488 antibody.
The presence of mouse IgG was detected by the same secondary anti-mouse antibody (fluorescein isothiocyanate/FITC-tagged donkey anti-mouse IgG, Jackson ImmunoResearch Laboratories, USA) used to visualise the primary anti-β dystroglycan antibody which was raised in mouse, explaining the presence of both β dystroglycan and endogenous mouse IgG on the same brain sections.
To ensure equal protein loading across the gel, membranes were submerged in stripping buffer (62.5 mM Tris/HCl, 100 mM 2-mercaptoethanol, 2% (w/v) SDS, pH 6.7), and incubated at 50 °C for 40 min, and re-probed with a polyclonal control CREB antibody, or the anti-tubulin antibody, followed by detection with the same secondary antibody.
Nevertheless, this raises an important question as to why the endophyte needs to stimulate the host plant to produce Taxol when the endophtye can produce the same secondary metabolite by itself, at least in vitro[ 16].
Alternatively, strains that appear different by morphology and 16S sequencing could produce the same secondary metabolites.
The girl's real identity was revealed by chance in February 2015 after her younger biological sister began attending the same secondary school.
He went to the same secondary school as his missing brother and his surroundings are a constant reminder of the once happy family now scarred by war.
