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Today, nanotechnology is monitored and regulated by the same procedures as other products and medicines.
The first generation cells were prepared by the same procedures as the second generation cells except the AFL coating.
The GPR40 transactivation assay was performed by co-transfecting HEK293 cells with Peak13-CD5L-hGPR40, pCMV-Gal4-hElk1, Peak12-Gal4UAS-luci, and pRL-TK, followed by the same procedures as described above for the GPR119 transactivation assay.
Method B: By the same procedures as described in making compound 5 using compound 4 and triethylsilane as reactants.
The evaluation of the antioxidant capacity was also performed using DPPH and FRAP methods by the same procedures as for the tea infusion.
To confirm the specific differential expression of genes by other means, we quantified a small set of relevant gene transcripts by RT-PCR in total RNA extracted from the same cell preparations by the same procedures as for microarray hybridization.
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By doing the same procedures as steps 1-3, we can prove that (3.17) holds for all (alphageq0).
Thus, using the structural bioinformatics tools [23], the 3D structure of the H1N1-M2 (GQ385383) channel was developed by following the same procedures as elaborated in references [24] [26].
This protein is separated by IEF along with the other strips, and is loaded on the polyacrylamide gel by using the same procedures as for the target proteins.After running the second dimension, proteins are transferred to a PVDF membrane, and the proteins are probed with specific antibodies.
Total RNA from each region of interest was isolated by microdissection following the same procedures as for microarray analysis, and primers for ferret gene homologs were designed based on the same ferret-specific sequences.
The [C]-benzoyl-CoA and [C]-benzoic acid were separated on an analytical C18 column (RESTEK) by HPLC using the same procedures as described above, and then quantified using an in-line β-RAM4 (Lab Logic) scintillation counter.
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