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Likewise, for the fluorescing DNA stain 4′,6-diamidino-2-phenylindole (diamidineso a diamidine, resistance factors of 32.0 and 31.5, respectively, were displayed by the same mutant cell lines (Table 1).
Similar(59)
The essential features of the diurnal activity profiles were not a function of the specific lighting cycles as shown by testing the same mutant combinations under different LD cycles.
The inhibitory behavior of decylubiquinone is consistent with its inability to be utilized for proton translocation by the same mutants (Figure 6).
The requirement of initially low and then gradually increasing R concentrations to select for mutant M296I [37], and the failure to select the same mutant by subjecting the same virus to 5 mM R, may relate to suppressive effects of the FMDV quasispecies when populated by higher frequencies of mutant genomes generated under elevated mutagenic intensities [71], [73].
Small genetic variants, including SNPs, single base pair or small indels (S-indels) and small complex events in each sequenced sample were called at positions covered by sequences of both the forward strand and the reverse stand, and by at least three reads supporting the same mutant allele.
Laccase properties were compared to those obtained for the same mutant enzyme expressed by S. cerevisiae.
These strains carry the same mutant alleles as described by Bekker et al. [37].
To confirm that all mutants classified in the same mutant line had an identical DNA mutation, all of the mutants derived from the same batch were confirmed by PCR and sequencing analysis.
The second method detects purR mutants selectively by using the same mDEL1 mutant (TT26174) grown to saturation at 30° in 2.0 ml NCE with 0.2% glycerol and the appropriate supplements.
Taken together, the results from these experiments show that H3-L61W and H4-R36A confer similar phenotypes associated with transcription and chromatin defects and that one of these phenotypes (that seen in the context of the FLO8 - HIS3 reporter construct) can be partially suppressed by the same two Spt16 mutants, albeit at varying levels of efficacy.
In the mix B, the EG I was replaced by an equal amount of endoglucanase rCelStrep; in the mix C the LArb was replaced by an equal amount of the α-l-arabinofuranosidase rPoAbf; in the mix D the LArb was replaced by the same amount of the mutant rPoAbf F435Y/Y446F.
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