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This cut-off point was chosen by progressively lowering the cut-off until further lowering it resulted in multiple clusters driven primarily by the same modification(s).
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Furthermore, an avidin with two specific ligand-binding sites was created by applying the same modification to dcAvd.
The samples were subsequently bisulphite-converted using the EZ DNA Methylation-Gold Kit as recommended by the manufacturer (Zymo) with the same modification to cycling conditions described above.
In type I PKSs, such MT domains are often employed by noniterative trans-AT and by iterative enzymes to methylate the growing polyketide chain at the α-position, whereas noniterative cis-AT enzymes achieve the same modification by using methylmalonyl-CoA in place of malonyl-CoA as extender unit [ 32, 34].
To document the specificity of the antisense S-oligos we used appropriate scrambled (scr) control S-oligos with the same modification custom-prepared by GeneLink (antisense 5′-A*C*G*UGACACGUUCGGA*G*A*ATT-3′; sense 5′-U*U*C*UCCGAACGUGUCA*C*G*UTT-3′).
Air France's Concordes did not have the same modification.
Any modification of the original half results in the same modification on the mirrored half.
Moreover, the same modification of an entity can be represented on the map at several places by multiple aliases.
The system of moment equations (16) can be derived from the last system for particular probability density functions by using the same modifications as in the proof of Theorem 1.
Post-translational modifications of Syt1 were analyzed by mass spectrometry, revealing the same modifications of Syt1 that were previously described for Syt1 purified from brain extract or mammalian cell lines, along with a novel modification of Syt1, tyrosine nitration.
Experiments were performed according to the method of Randall and Selitto [ 5], with the same modifications used by Winter and Flataker [ 6].
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