GSH was determined from the same extract as P phy, by adding 0.2 M potassium phosphate buffer (pH = 8.0) and 10 mM DTNB (5.5′-dithio 2-nitrobenzoic acid)) and measuring the absorbance at 415.5′-dithio 2-nitrobenzoic
This way, a comparison of the nucleic acids extracted by the same extraction procedure from the different matrices, i.e. biscuit, feed and CRM 1%, is readily available.
Total flavonoids were determined in the same extracts by a reaction with AlCl3 at a basic pH, as described by Zhishen et al. (1999), with catechin used as a standard.
In the present study, the extraction was carried out under ultrasonication using 95% ethanol as the solvent, followed by fractionation of same extract with various solvents with increasing polarity and the final extracts were designated as NafE, NafEHx, NafEEa, NafEBu, NafEIn, and NafEW.
*Tentatively identified The same extracts were analyzed by LC-MS in order to obtain more detailed information concerning the differences in polar metabolite levels due to treatment.
For comparison, the same extracts were also cleaned by GPC.
Four of the identified chemotherapy response candidates, CSTB, F13A1, ISYNA1 and S100A13, were selected for technical validation by immunoblotting, using the same protein extract as in the MS analyses.
The relative abundance of silver stained actin was validated by western blot assays on the same tissue extract (Fig. 1).
This workflow was firstly evaluated by analyzing two aliquots of the same protein extract from normal human liver tissue.
However, "Lipid Metabolism, Molecular Transport, Small Molecule Biochemistry" became the top network involved when IPA was reanalyzed to include proteins that were previously shown to be differentially secreted by HepG2 cells treated with the same fruit extract [ 7].
