Sentence examples for by the same buffer from inspiring English sources

To distinguish an actual sensor response caused by interactions between the target and the immobilized probe from that caused by occasional fluctuations of medium refractive index, it is necessary to wash the flow cell before and after each sample by the same buffer solution and only then to determine a value of the SPR response.

To distinguish an actual sensor response caused by the interactions between the target and the immobilized probe from that caused by the occasional fluctuations of medium refractive index, it is necessary to wash the flow cell before and after each sample by the same buffer solution, and only then to determine a value of the SPR response.

On the first column, the mixture was loaded in 2 M urea, 0.15 M NaCl, 0.1 M Tris-HCl (pH 7.4) and eluted by the same buffer.

Embryos injected with MOs were dechorionated at 8 hpf, fixed with 2% fresh paraformaldehyde and 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH = 7.4) at 8.5 hpf for 2 h at room temperature, washed by the same buffer, and then post-fixed with 1% OsO4 in the same buffer for 2 h on ice.

The pellets binding to Avicel were washed twice by the same buffer at 4°C and were centrifuged.

Binding protein was eluted by the same buffer, the eluted protein was pooled and loaded on an anion-exchange Mono Q column which was equilibrated with 20 mM Tris HCl buffer (pH 8.2).

Total protein extractions for all enzymatic analysis (activity and isozyme assays) were performed by using the same buffer except ascorbate peroxidase (APX) assays.

Control sections were applied by ussing the same buffer but omminting the primary antibody.

For ACE, the proteins in the BGEs were diluted to appropriate concentrations by using the same buffer.

Proteins were eluted by using the same buffer (0.01 M HEPES + 0.15 M NaCl) added of 0.05 M GalNAc (AppliChem, Darmstadt, Germany).

Non exposed insects were treated side-by-side with the same buffer.