Sentence examples for by the rtPCR protocols from inspiring English sources

Then these samples were used to detect Y. pseudotuberculosis and pathogenic Y. enterocolitica by the rtPCR protocols described before.

Tonsil samples, collected during the 2010 2011 hunting season, were analyzed by direct rtPCR following the protocols described below.

Isolates reactive by the screening RTPCR were subjected to penA DNA sequencing as described previously [ 18].

Overall, 59/684 isolates were reactive by the screening RTPCR, indicating the presence of a mosaic penA allele.

Of isolates reactive by the screening RTPCR, 15/59 displayed an alert value CRO MIC and 21/59 displayed an alert value CPD MIC.

Both isolates that displayed alert value AZM MICs were non-reactive by the screening RTPCR and had non-alert value extended-spectrum cephalosporin MICs.

The use of densitometric analysis of PCR results qualitatively matched the rtPCR results for all the antibodies used here since all above mentioned known targets were detected above background by both densitometry and rtPCR (Figs. 2 and 3).

The RTPCR was carried out using iCycler (Biorad, Hercules, CA).

After amplification of the viral genomes by M-RTPCR, the sequencing of the PCR products resulted in approximately 184,000, 248,000, 214,000 and 192,000 viral short-reads, respectively.

The analysis of variance showed significant differences in Yersinia detection by rtPCR according to enrichment protocol used (p = 0.0141).

The analysis of variance showed significant differences in Yersinia detection by rtPCR according to the enrichment protocol used.