Sentence examples for by the reverse transcriptase from inspiring English sources

Oligonucleotide primers were designed and used to amplify the VH and VL regions of guinea pig RNA by the reverse transcriptase polymerase chain reaction.

Significant up-regulations in the mRNA expression of adhesion signaling molecules occurred with the fusion protein link, as analyzed by the reverse transcriptase polymerase chain reaction.

The high number of errors made by the reverse transcriptase (RT) enzyme and the absence of an RT proofreading mechanism during HIV-1 replication leads to new antigenic variants that escape current immunological attack.

Bonhoeffer et al. suggested that template switching by the reverse transcriptase acts as a repair process to deal with breaks in the ssRNA genome.

Briefly, a first cDNA synthesis was performed with oligo dT primers supplied by manufacturer, and dC residues were introduced by the reverse transcriptase when it reached the mRNA end.

In brief, first strand cDNA was synthesized by the reverse transcriptase SuperScript™ II from ∼600 ng of total RNA using 2.5 pmol of the PEG10 gene-specific primer KIAA-3'OUTER.

Negative controls were made by replacing the reverse transcriptase with diethyl pyrocarbonate-treated water.

cDNA was obtained using the iScript Select cDNA Synthesis Kit Bio-Rad Laboratories ABB, Stockholm, Sweden), and the absence of DNA contamination was verified by excluding the reverse transcriptase from subsequent PCR reactions.

Controls were performed by omitting the reverse transcriptase enzyme.

Negative controls were performed by omitting the reverse transcriptase (not shown).

For each RNA specimen, a negative control was prepared by omitting the reverse transcriptase.