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For all organs except bone, the image analysis and residence time calculations were performed by the reported methods with some modifications [35,38].
Several routes for imidazo[1,2-a]pyridine and imidazo[1,2-a]pyrmidine skeletons have been developed [23 31], but, the access by the reported methods to those functionalized fused systems, is usually difficult.
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The antibacterial activity of compounds 3a-3k against R. solanacearum was assayed by the reported method [30].
The isatin Mannich bases, namely 1-morpholinomethyl-3 1-N-dithiooxamide iminoisatin [MMTOI] and 1-morpholinomethyl-3 1-N-dithiooxamide iminoisatintin 1-morpholinomethyl-3 1-N-dithiooxamide iminoisatin[1-morpholinomethyl-3 1-N-dithiooxamide iminoisatin
The sodium salts of dithiocarbamates were prepared according to the following reaction by the reported method [25]: R 2 N H + C S 2 → 4 ° C NaOH Ethanol R 2 C S 2 Na.
As directed by the reported method [20], a PEG Puro spacer was synthesized on a large scale by Invitrogen Japan K.K., Tokyo, Japan and Takara Bio Inc., Otsu, Japan.
SOD was measured by the reported method [ 21, 24].
Catalase was measured by the reported method [ 21, 23].
Since the dried extract contained mainly flavonoid glycosides, it was standardized in its flavonoid glycosides content (4.6%) by the reported method [ 21].
All the plant samples were then frozen in liquid nitrogen before total RNA extraction and first strand cDNA synthesis by the reported method.
Carbonic anhydrase inhibition was measured by the reported method [ 29] after standardization of reaction conditions such as concentration of enzyme and substrate, buffer pH, and duration of reaction.
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