Sentence examples for by the relative quantification from inspiring English sources

After evaluating RNA integrity, complementary DNA was synthesized, followed by the relative quantification each of the genes of interest.

The resulting data were analyzed by the relative quantification method using as calibrators either the low expressing control cells H441 or the parental monocytes (Fig. 1A and 1B).

Data were analyzed by the relative quantification (ΔΔCt) method, as described in our previous studies.

Expression levels were calculated by the relative quantification (RQ = 2−ΔΔCt) method [ 26].

Relative expression ratios were calculated by the relative quantification real-time-PCR method [ 55].

Expression of target genes was evaluated by the relative quantification method.

To quantify the abundance of anammox 16S rRNA in the bacterial populations of both reactors, we performed real-time PCR assays by using the relative quantification method and the digestate as calibrator).

Data were normalized by β-actin gene and the relative quantification was made by the delta Ct method (Applied Biosystems, Foster City, CA, USA).

The change of the expression levels of these transporter genes, membrane proteins and regulators is presented by Figure 1 as the relative quantification of the expression level in the TET induced resistant AG100 strains relative to wild-type AG100 grown in the absence of TET at each stage of the induction process.

CT values were calculated automatically by the software and the relative quantification was determined using the ∆∆CT method.

A mathematical model introduced by Pfaffl was used for the relative quantification of target genes [ 22].