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The standard deviation of the receiver DCBs estimated by the relative method was smaller than that of the single method.
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In addition, we show by example that the relative method outperforms the absolute method if the calibration process is imperfect.
Quantification of gene expression was carried out by the relative quantification method (2-∆∆CT method) [ 53] implemented in StepOne software v2.3.
In Figure 9d, we report the corresponding composition image obtained by the relative intensity method.
As explained previously these local weights of each PEI are evaluated by the relative evaluation method of comparative judgments that is pairwise comparison in AHP.
The resulting data were analyzed by the relative quantification method using as calibrators either the low expressing control cells H441 or the parental monocytes (Fig. 1A and 1B).
Expression of target genes was evaluated by the relative quantification method.
Expression levels were calculated by the relative quantification method (ΔΔCt) using the CFX Manager 2.1 software (Bio-Rad).
The reaction was run using System 7300 (Applied Biosystems, Foster City, CA, USA) and results were analyzed by the relative quantity method.
Expression levels of target genes were determined by the relative quantification method using β-actin as an endogenous control.
The mRNA level was analyzed by the relative quantification method based the Ct values obtained from the gene of interest and β-actin (the internal control).
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