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For comparative analysis, ELISA intensities of all strains were divided by the reference strain C. difficile 630 ELISA intensity.
To calculate viral fitness scores, copies of DNA produced by test strains were divided by copies of DNA produced by the reference strain at a coincident time point post-infection [Fitness score = (HIV-1 DNAT/HIV-1 DNAR)], where HIV-1 DNAT and HIV-1 DNAR correspond to copies of HIV-1 DNA produced by the test and reference strains, respectively.
Mutants Δtsp3::hyg or Δtpl1::hyg caused foliar lesions identical in number, size and aspect to those induced by the reference strain P1.2- Δku80::bar.
We observed a slight increase in succinate production in the mutant strain; yield of 0.0033 g/g-glucose was obtained in anaerobic growth conditions whereas no (or undetectable amounts) succinate was produced by the reference strain.
Wells of polystyrene plates were coated with a predetermined optimum quantity of positive and negative antigens prepared on a Vero cell culture infected by the reference strain ISS Phl3 kindly provided by the Bunyaviridae Laboratory (Professor Bouloy, Institut Pasteur de Paris), in phosphate-buffered saline (PBS) (pH 7.2) and incubated overnight at 4°C.
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By incorporating the reference strain, the application range of the strain-based failure assessment diagram method is extended to ECA of severe circumferential surface cracks in reeled pipes.
To investigate the occurrence of intergenic recombination events in S. cerevisiae, we first identified paralogous genes by BLASTing the reference strain S288c's proteome against itself.
The MIRU-VNTR 24-loci profiles and spoligotyping patterns were used to classify the strains into main phylogenetic lineages by using the reference strain collection and identification tools available online at http://www.miru-vntrplus.org[ 21].
Single deletion mutants were constructed by crossing the reference strain a ura215,30,72 his3Δ 200 trp1Δ 63 (aUHT) with strains from the EUROSCARF deletion collection (Y16546 (Δ yku80), Y11781(Δ lig4), Y10540 (Δ rad52) and Y13756 (Δ rad59)).
Poisson regressions were also fitted to the number of perithecia produced by the reference strains when crossed with the evolved strains.
These crosses also made it possible to evaluate the male fertility of each of the evolved strains: its ability to induce the production of perithecia by the reference strains.
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