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The masked SNPs were then imputed from the genotype information provided by the reference population.
Whilst absolute Z-scores may be influenced by the reference population, changes are more likely to be preserved.
The cutoff value for low FFM was defined as FFMI below the 10th percentile and obesity was defined as FMI above the 90th percentile, as defined by the reference population [ 40].
For across-population genomic prediction, the genetic correlation between populations has to be taken into account, because it limits the part of the genetic variance in the selection candidates that can be explained by the reference population.
The differences in sensitivity and specificity rates may be explained by the reference population, adopted cut-offs from various references, methods used to assess %BF, and the cut-offs for excess fat [ 31, 33].
However, in agreement with most studies using other approaches and not adjusting by the reference population [ 5, 27- 32], our finding of lower emergency department utilisation by immigrants suggests overall lower healthcare use in this population.
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Therefore, the increase in accuracy of genomic prediction achieved by enlarging the reference population, as shown by e.g. [ 1- 3], might not only result from a more accurate prediction of SNP effects, but also from capturing a larger proportion of the alleles that segregate in the complete population.
This means that imputation accuracy could be increased by increasing the number of HD genotypes and by improving the reference population to maximize its relationship with the population to impute.
We calculated the coordinates of each hybrid zone specimen on the discriminant axis defined by the reference populations (figure 2).
Similar results were found for occupational health practitioners, who were contacted by 3% of ambulance personnel but by 24% of the reference population.
This proportion of the genetic variance in the selection candidates captured by SNPs in the reference population is the maximum accuracy of genomic prediction for those populations based on the used SNP chip [ 39].
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