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The number of reads matching each genomic object harbored by the reference genome was subsequently computed with the Bioconductor-GenomicFeatures package (Carlson et al. 2011).
Some smaller CNVs and those in regions not as well mapped by the reference genome may have gone undetected.
Functional groups used to classify CDS were defined by the reference genome annotations and based on COG v1.0 / CDD groupings.
Our algorithm starts by only considering the matepairs that are unlikely to be explained by the reference genome.
All of the remaining matepairs are unlikely to be explained by the reference genome, and hence are potentially involved in a structural variant.
The NEEDLE alignment shows that the ORF in the target genome is truncated at the N-terminus but contains the remaining 240 aa encoded by the reference genome.
Similar(51)
One way of addressing this problem is to begin by annotating the reference genome with all the putative SNPs generated by different discovery algorithms.
We adopted a bioinformatics-based approach, and by utilising the reference genome sequence of B. pseudomallei K96243, we identified 12 putative OmpA genes in the genome.
M indT heG ap detects insertion sites by scanning the reference genome and testing membership of reference k-mers in the de Bruijn graph.
The RNA-seq paired-end reads were mapped into transcripts using TopHat by setting the reference genome as S. cerevisiae (sacCer3, UCSC).
RNA-seq can provide a more comprehensive and accurate transcriptome analysis of cotton fiber development by using the reference genome sequence of Gossypium raimondii Ulbr.
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by the reference dna
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by the reference phase
by the reference cantilever
by the reference laboratory
by the reference rater
by the reference code
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by the reference point
by the reference standard
by the reference structure
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