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These complex protein-protein interactions [2], [4], [46] are also likely to regulate the kinase activity of ILK, as indicated by the recent structure determination of a complex between the ILK kinase domain and the C-terminal calponin homology (CH2) domain of α-parvin [47].
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This module might encompass CCs of widely different lengths, as suggested by the recent structures, but are unified by the formation of a C-terminal 4-helical bundle to which the kinase catalytic domain is connected.
Binding of parvins was wrongly assumed to activate ILK, as the recent structure by Fukuda et al. revealed that α-parvin makes use of the active site of ILK for binding [ 36 ].
This observation is further supported by the more recent structure determination of a homologous prokaryotic ion channel (7).
Best characterized is the direct interaction of Maf1 with the N-terminus of Rpc160, shown first in vitro (Oficjalska-Pham et al, 2006) and greatly enhanced by the recent crystal structure of Maf1 and cryo-electron microscopy structure of Maf1 bound to Pol III (Vannini et al, 2010).
This activity has been rationalized by the recent crystal structure of yeast CRM1 bound to RanGTP and RanBP1: RanBP1 induces a conformational change in the Ran-interacting HEAT-9 loop of CRM1, leading to constriction of the NES-binding groove and consequent release of the NES [33].
This method is supported by the recent ssNMR structure of the GPCR CXCR1, which was derived using a similar protocol.
A new view of the FA pathway has been provided by the recent crystal structure of the mouse FANCD2 FANCI complex (Joo et al. 2011).
This model is also supported by the recent crystal structure of unphosphorylated human Ire1α, which shows human Ire1α in an inactive conformation.
Our model of the pUb-binding site along helix H3 of RING1 is confirmed by the recent crystal structure of pUb bound to Pediculus humanus R0RBR parkin (Wauer et al, 2015).
Latter result is confirmed by recent structure data [39].
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