Sentence examples for by the published sequences from inspiring English sources

Although most of the euchromatic regions (95%) of the rice genome were covered by the published sequences, 62 gaps and heterochromatic regions, centromeres, and telomeres, corresponding to 5% of the total genome, remained unrevealed.

This primer was designed on the basis of the conserved region of Adh, which was determined by comparing the published sequences of Adh [ 22].

Primers for all the primates were designed by aligning the published sequences of human (Esembl ID: ENSG00000047634) and macaque (Ensembl ID: ENSMMUG00000012899, Ensemble genome browser [ 24]).

To avoid biases introduced by missing data from the published sequences, we used a fragment (corresponding to ca. 95% of SSU rRNA) spanning from helix 5 until 2 bp before helix 50 (i.e. positions 48 1896 in Amphimedon queenslandica), and only considered the 123 sequences without data missing within this region (listed in Additional file 4).

The cloned UGE open reading frames were confirmed by sequencing to be identical to the published sequences (see Figure  1 for accession numbers).

The promoter region of HSP70 was amplified by PCR according to the published sequence [ 25] and inserted into pGL3-basic luciferase reporter vector (Promega, Madison, WI).

Our sequence (GU936969) differed from the published sequence by three amino acids, located in the extracellular amino-terminus, the second transmembrane domain and the fourth transmembrane domain.

Following bead-based recovery of the biotinylated product, a nested amplification was performed using an adapter primer modified from the published sequence by the addition of a single selective base at the 3'-end of the primer.

Additionally, fragments of the S, M, and L segments from isolates of DUGV collected in 1999 from the Nairobi abattoirs were sequenced by using primers designed from the published sequence of DUGV (3 ).

Probes were generated by reverse transcription-PCR based on the published sequence.

Presence of classical, El Tor, and Calcutta type rstR genes of CTX phage were also determined with PCR by using specific primers derived from the published sequence of the respective genes.