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The IC50 of BF4 − determined here (4.7 μM) and previously (1.6 μM [17]), as an inhibitor of hNIS in vitro suggests that the quantity of 19F-BF4 − in the radiopharmaceutical prepared by the published method [15] and administered in a dose sufficient for high-quality PET imaging could be in the range capable of adversely affecting radiotracer uptake via NIS.
Cepstral derivatives are obtained by the published method [32].
The zymogram analysis was performed by the published method of Zhang et al. [ 20] with birchwood xylan replaced by beechwood xylan (Sigma).
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To predict the virulence of a species/strain/isolate by its gene/protein sequence, the published method for virulence evaluation of low and high pathogenic avian influenza LPAI and HPAI [ 53] was implemented in the system.
The radiosynthesis of the thymidine analog (F-FLT) was performed according to the published method by Grierson and Shields [ 18].
The F-labeled nitroimidazole compound fluoroazomycin arabinoside (FAZA) was performed according to the published method by Piert et al. [ 7].
This paper contributes to knowledge by comparing the published methods, ranging from well-known simulated annealing to recent published iterated local search, and evaluating the six encoding schemes, 30 decoding procedures and five objective functions on the performances of the meta-heuristics meanwhile.
It is likely that Hoffman started by recreating the published methods.
The genealogy can be sampled by any of the published methods available in BEAST [ 24].
Some of the limitations of the method proposed in this study are shared by most of the published methods: marker alleles are assumed to be selectively neutral, mating within the population is at random and immigration from other populations is absent (Leberg 2005).
The results of motion estimation using proposed variants seems to be faster than the published methods by maintaining similar peak signal to noise ratio.
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