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The samples were converted to methyl ester derivatives by the protocol reported by Lepage and Roy (1986).
Affinity-based ELISA was performed by the protocol reported earlier by Barak et al. 2005 [ 47].
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The AROS model was subjected to molecular dynamics simulations by using the protocol reported in the Methods.
Gliadins and glutenins were extracted and quantified by RP-HPLC following the protocol reported by [ 24].
In addition, all patients in both groups were managed by tight glucose control, using the protocol reported by Van den Berghe [32],[32].
In addition, all patients in both groups were managed by tight glucose control, using the protocol reported by Van den Berghe [ 32],[ 33].
The insertion mutants were screened based on the protocol reported by Heap [24], Intron insertions were verified by PCR using primers Cac1494B and Pex1494E flanking the target site and then PCR products were purified and sequenced, the sequence has been deposited in (Genbank number: HQ257448).
After confirmation by mini-preparing plasmid DNA samples from the Agrobacteria followed by restriction enzyme digestion, the transformed Agrobacteria were used for rice transformation according to the protocol reported by Hiei et al. [ 55].
Isocitrate lyase activity was assayed according to the protocol reported by Dixon and Kornberg (glyoxylate phenyl hydrazone formation) [42] at 10 μM of the compounds.
The glycerol release was measured essentially according to the protocol reported by Laurell et al. [93].
C. acetobutylicum SMB009 was screened according to the protocol reported by Heap [30].
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