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The supernatant was quantitated in a mass spectrophotometer by the protocol described by Wright and Upadhyaya (1998).
Differentiation of H9 hESC into hNSC was followed by the protocol described previously (Liu et al., 2012).
A quality evaluation modified by the protocol described by Antczak [46] and Jadad [47] was performed for each article.
Microtiter wells were coated with the α-synuclein antigens at concentrations of 15.0 and 20.0 µg/ml of monomers and oligomers, respectively, in 50 mM phosphate buffer, pH 8.0 4°CC) and assayed by the protocol described in [8], [9].
Immuno-labeling was initiated by de-paraffinization and blocking the sections for 1 h with TPBS, followed by the protocol described below.
Two weeks later, these mice were intraperitoneally injected with adenanthin (10 or 20 mg/kg) or saline as control by the protocol described in materials and methods.
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The sample collection techniques and transportation procedure was done by following the protocol described by Hossain et al. (2015).
Chromatin immunoprecipitation (ChIP) analysis was done in Ramos and MUTU-I celinesnes by following the protocol described by [38].
A paw edema assay was performed by using the protocol described by [ 21].
Alkaloids content was measured by following the protocol described by Harborne [ 29].
MIRU typing was performed by using the protocol described by Cowan et al. (22 ).
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