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This development is regulated by the precise expression of genes in the right cell type and at the right time, and this regulation is mediated by the synergistic/antagonistic action of a limited number of transcription factors.
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Apart from new engineering strategies, a better understanding of the microRNAs' roles in determining phenotypes could also lead to the development of novel screening tools that allow better prediction of cell behavior at industrial scale, by analyzing the precise expression pattern of those microRNAs that were previously identified to control these properties.
The precise expressions can be found in the Appendix.
This idea is also supported by the precise gene expression response that we observed between the T4 treatments: essentially all of the genes were expressed in the same direction, and a subset of genes that could be reliably compared showed the same magnitude of gene expression.
For populations of fixed size, as can be seen from expression (1) or the more precise expression (10) obtained by Kimura [ 19] and Moran [ 20], the fixation probability is a monotonically increasing function of the sole relative fitness.
Each gene has its own optimum "binarization threshold" to distinguish when it is "on" or "off," which is not clearly defined, and even if it was, significant information will still be lost by not accounting for the precise intermediate expression levels.
Temporal expression of transcripts at the genome level during the growth-phase was obtained by HTS (Figure 4) whereas precise expression of a subset of 26 sRNAs identified in this study was assessed by RT-qPCR.
We used homologous recombination to produce a GFP reporter for the analysis of endogenous, in vivo, levels of ADGF-A expression by the precise replacement of the ADGF-A coding sequence with that of GFP (Figure 1).
We obtained very precise expression profiles for the cytokines and proteinases under investigation by using real-time PCR.
To further understand the function of poplar GS genes, the precise gene expression patterns were determined by in situ hybridization and laser-capture microdisection analyses.
The language of rights can give precise expression to elaborate structures of freedom and authority.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com