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MicroRNAs (miRNAs) are produced by the precise excision of an approximately 21-nt miRNA/miRNA* duplex from the stem of a single-stranded, stem-loop RNA precursor [3].
In plants, miRNAs are obtained by the precise excision of ~21 nt smRNAs from the stem of a single-strand, stem-loop precursor.
The phenotype was reverted by the precise excision of the piggyBac transposon or transgenically-expressed CG6750 (data not shown); this indicates Rh1 reduction is caused by reduced CG6750 gene function.
DNA transposition by piggyBac superfamily transposases is distinguished from most other DNA transposon superfamilies by the precise excision of the transposon from the donor site and preference for insertion in TTAA sites (Cary et al., 1989; Fraser et al., 1995).
Alternatively, the two Helitrons could have inserted in reverse order, followed by the precise excision of Helitron No.1 in a common progenitor of modern maize lines having only Helitron No. 2 at the insertion site.
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The precise excision, HtrA2 Δ1 and HtrA2 Δ1 rescued flies were tested by genomic PCR, RT-PCR, and western blot to confirm the deletions and the absence of both transcript and HtrA2 protein in mutants.
Therefore, the precise excision of these P-elements reversed the hypoxia tolerance phenotype.
However, a role of the integrase and excisionase genes for the precise excision and integration of the PAI cannot be ruled out.
Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences.
While this worked for the precise excision allele ald excision-25, in more strongly compromised genotypes it proved difficult to confidently determine spindle orientation by this method.
Results of the PCR and sequencing demonstrated the precise excision of the GIYep-1 island.
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