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When cells have not yet established well-defined adherens junctions, as shown by the poor expression of E-cadherin at cell cell contacts (left panel), PrPc was mainly detected intracellularly.
The large-scale production of recombinant pharmaceutical proteins is often hampered by the poor expression of their mature, active forms in prokaryotic hosts such as E. coli and by the high costs and the limited scalability of traditional fermenter-based platforms using mammalian cells.
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This is in agreement with the poor expression observed by immunofluorescence.
That the poor expression of MMAC/PTEN in pancreatic cancer cells could be due to promoter methylation was indicated by methylation-specific PCR analysis.
However, the microbial potential of sesquiterpene production is limited by the poor heterologous expression of plant sesquiterpene synthases and the deficient FPP precursor supply.
Importantly, the observed lack of 1-indanone conversion is not caused by a poor expression because all PAMO variants were equally well expressed under these experimental conditions (data not shown), thereby pointing towards a low reactivity of PAMO P440L and P440G with 1-indanone like the wild-type enzyme and possibly a lowered uptake of the substrate under non-optimized conditions.
In particular, the liver sections of group 3) were characterized by poor expression of VEGF in endothelial cells that located in the wall of the vena centralis and sinusoids.
This issue is further complicated by poor expression of HIV structural antigens due to rev dependence.
However, different dual-gene expression vector strategies are limited by poor expression of one or both transgenes.
We conclude that expression of correctly folded and targeted aquaporins is strongly dependent on recombinant gene dosage and also that increasing the gene dosage could, to some extent, help improving poor expression caused by inefficiency at the transcriptional and translational levels.
To investigate whether the poor AtSIP1 1 expression was caused by low transcript levels an mRNA quantification experiment using qPCR was performed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com