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CO2 production was calculated according to the single pool equation of Speakman [24]:
Dilution spaces for oxygen (N0) and hydrogen (Nd) were calculated by the plateau method (Speakman and Krol, [ 59]).
kc (min−1) was multiplied by the total body bicarbonate pool Nc (mol) as calculated by the plateau method [ 7], and converted to carbon dioxide production rate (; ml min−1) by multiplication with 22.4 l mol−1.
We confirmed that R dilspace determined by the plateau method ranged between 1.026 and 1.043 in our data, which was within the expected range; therefore, the analytical error in our results was negligible (Speakman, 1997).
The dilution space ratio (R dilspace) determined by the plateau method ranged between 1.026 and 1.043 (n = 4, Table 1), which was within the range found for other taxa (Speakman, 1997).
Therefore, in our study, N o and R dilspace determined by the plateau method were used for the calculation of metabolic rates using the one- and two-pool models.
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Deuterium and 18O levels in urine samples were quantified by mass spectroscopy and the values were used for calculation of total energy expenditure according to the plateau method (Schoeller, 1992).
Then, the threshold α may be adjusted automatically to the data set, so that the resulting cores are stable, e.g., by means of the plateaus method [39].
The slow evolution is justified by the plateau regions presented by the MSE surface.
The GFR was calculated by dividing the infusion concentration by the plateau concentration.
When both response plateaus are present, the parameter values generated by SDRS are almost identical to those generated by the iterative method (Table 1 and Supplementary Figure S2).
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