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The diagnosis of NSCLC was made by the pathologist based on histopathological examination.
The primary causes of death were assigned by the pathologist based on integration of the clinical information and the gross and microscopic findings from the autopsy.
It was classified as strong or weak by the pathologist based on a composite assessment of the percentage of the visualized field as well as the intensity of immunohistochemical reaction specifically in Barrett's epithelium.
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The TRG of the primary tumor was determined by the same pathologist based on the tumor regression grading system initially described by Dworak et al. [ 14].
The staining results were evaluated by a pathologist based on the percentage of staining in tumor cells.
Third, we also used as a 'regulator' a clinical parameter, the Gleason grade, a discrete score assigned by a pathologist based on the microscopic appearance of prostate tissue biopsies.
The immunohistochemical intensity was semi-quantitatively scored by an experienced pathologist based on the intensity of the Bmi1 staining as described previously [ 35].
Liver histology was graded and staged by a pathologist based on the international classification [ 9].
Each sample was histologically assessed by a pathologist based on the classification criteria followed by WHO [ 11].
From representative donor paraffin blocks of the primary tumors, tissue microarrays were constructed by transferring tissue cylinders of 0.6 mm (3 cylinders per tumor) from the tumor area, determined by a pathologist based on haematoxylin-eosin stained slides, using a tissue arrayer (Beecher Instruments, Sun Prairie, WI, USA) as described before [ 27].
Adjacent noncancerous and OSCC tissue samples were selected by a pathologist based on diagnosis and microscopic morphology.
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