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None of the them were affected by the pH difference.
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The pH difference, ∆pH (final pH initial pH) vs initial pH was plotted to determine the pHpzc.
By adjusting the pH, differences between native or mutant major capsid can be offset assuring hydrogel assembly with either phage system.
Considering the pH differences in the body, various drug delivery systems can be designed by utilizing smart biopolymeric compounds with the required pH-sensitivity.
The pH differences (ΔpH = pH purified sample) – pH reference)) are within ±0.0004 pH units over a pH range between 7.4 and 8.2.
A permeant acid stresses the cell by importing protons, depressing cytoplasmic pH, and by concentrating the organic anion within the cytoplasm in proportion to the transmembrane pH difference (ΔpH) [12] [16].
In addition the peak symmetry caused by a conductivity difference is in most experiments counteracted by a pH difference.
The cytoplasmic pH was measured for 3 min before collapsing the transmembrane pH difference with 30 mM sodium benzoate.
Δc represents the combined intracellular concentration difference of bound and free protons, which is given by the product of intracellular pH difference, ΔpH, times the intracellular non-bicarbonate buffer capacity BF of 24 mM/ΔpH (see Methods).
The measured pH difference was negligible between all groups (pH 8.3 8.5).
In order to exclude that the results were biased by pH differences we also analysed the pH of the medium in hypoxia and normoxia after 24 96 h and found no differences in pH levels between the two conditions (data not shown).
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