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The distance between the realizations of an OTU in two samples was defined as an edit distance between the two corresponding consensus sequences normalized by the overlapping sequence length excluding the gaps.
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By comparing the overlapping sequence with the BRAF gene's reference sequence (NM_004333.4), we found that the mutation corresponds to c.1799_1803delinsAT (p.V600_K601delinsD) when expressing using Human Genome Variation Society-approved nomenclature [ 20].
We might like to call an overlap reliable if the overlapping sequence contains even one reliable 20-mer, but this might be an illusion caused by sequencing errors.
PCR products of PD1 and of Fc were fused by overlap-extension PCR, where the hinge region served as the overlapping sequence.
The overlap information label (General Info label) provides the internal id, chromosome name, the orientation, the overlapping phase, the overlapping length and the overlapping sequence.
The full-length cDNA sequence (453 bp) was completed by combining the overlapping sequences from each PCR product and then confirmed by a long distance PCR (Fig. 2C; GenBank accession No. AY396740).
The overlapping sequences were identical.
For A. queenslandica, sequences yielding tBLASTn values lower than 10−3 were retained, and the complete gene sequence reconstructed by retrieving overlapping sequence fragments.
In "The Wedding Complex: Forms of Belonging in Modern American Culture," Elizabeth Freeman briefly sketches the millennium-long history of marriage in the West: Marriage has been regulated — and weddings officiated — by an overlapping sequence of institutions.
The sequences were then assembled using the Cap3 Assembler downloaded from http://www.cs.iastate.edu/∼xqhuang/[37] to reduce redundancy and increase read reliability by condensing overlapping sequence and associated quality scores.
Redundancy describes the ability of overlapping sequences to minimize non-synonymous substitutions at the level of two or more proteins encoded by a single overlapping sequence.
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