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It also simulated L-LTP induced by the original protocol demonstrating synaptic tagging [32].
During this period approximately 60 individuals were identified as Recent Infection (RI), as defined by the original protocol [11].
As shown in Supplementary Fig. S2A D, the modified nanoHELP assay generated data having good correlations with the data obtained by the original protocol.
When E values from the 5 genes were averaged, a significant improvement of E following RNase H treatment was found: from -1.96 ± 1.10 to -3.43 ± 0.30 (P = 0.003) when using cDNA generated by the original protocol, and from -1.36 ± 0.70 to -2.88 ± 0.29 (P < 0.001) when using cDNA generated by the optimized RT protocol (Table 2).
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To solve this problem, it is necessary to perform a high-throughput second screening of candidate sequences, although this is not possible by using the original protocol of SAGE mainly because of the very short sequence tags generated.
The reasons for drop-outs and exclusion from the study were mainly change in the original protocol by either starting HRT by women originally assigned to the non-HRT group or vice versa, or lack of interest.
Various adjustments, all approved by ethics, have been made to the original protocol approved by ethics.
All mice were subjected to CLP surgery according to the original protocol by Wichterman et al. [19] with specifications made elsewhere [20].
For ARISA of bacterial and archaeal communities, the original protocol by Fisher and Triplett (1999) was applied in modified form as suggested by Weig et al. (2013), using 10 ng of DNA in a 12.5 µL PCR volume.
Fifty microlitres of cell extract was incubated with luciferase assay reagent based on the original protocol by de Wet et al (1987).
Following examples of other research groups that had applied the IM protocol before [ 57, 90], we modified the original protocol by restricting the amount and complexity of matrices in steps 1 and 2.
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