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The production of URAK by the optimized medium was 6326.98 FU/ml.
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HBF-3 production obtained by using the optimized medium was 4.52 g/l, which was in close agreement with the predicted value of 4.55 g/l.
This confirmed that the LiP activity could also be controlled by medium nutrition, and the optimized medium formulation was reliable for LiP production.
This validated that laccase activity could be controlled by medium nutrition, and the optimized medium formulation was reliable for laccase production.
As a result, total activity of HepA reached 20,650 IU l−1 in the optimized medium by a fed-batch mode in the 5-l fermentor.
The production of lovastatin by M. purpureus MTCC 369 in the optimized medium was found to be four times higher than the basal medium in the submerged fermentation.
Comparing to the lactic acid production in the medium with yeast extract as the only nitrogen source, lactic acid production in the optimized medium was increased by 30.4%.
Upon using the optimized medium and conditions followed by photocatalytic treatment an increase in decolorization of higher concentrations of the dye was obtained and a reduction in the COD was achieved (Table 4).
An experiment was performed under the predicted optimal conditions in order to validate the optimized medium.
Even higher enzyme activities were achieved by batch cultivations in a conventional stirred bioreactor on the optimized medium.
Glucoamylase production (26.3 U ml−1) in the optimized medium was in good agreement with the values predicted by the quadratic model (26.7 U ml−1), thereby confirming its validity.
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CEO of Professional Science Editing for Scientists @ prosciediting.com