Lagrange multiplier method is exploited for finding the optimized filter coefficients.
BMECs were purified by subculture on to either the standard 12-well Transwells (1.1 cm) or 24-well Transwells (0.32 cm) at the optimized filter seeding density of 1 million cells/cm, which yielded equivalent maximum TEER values.
Nevertheless, the optimized filter strategy of SiPaGene could outperform standard tools like SAM [ 6] and dChip [ 34].
It could be explain by the fact that the tungsten illuminant has a emission even lower in the range of 380−500 n m, which explains why the optimized filters are compensated.
Due to the predefined Gabor filters as an empirical selection for the CASIA-I dataset, the improvements caused by the optimized Gabor filters on the CASIA-I and CASIA-V4-Interval dataset are not so obvious.
Our approach permitted to generate a large and robust SNP datasets by the adoption of optimized filtering criteria.
The methods for optimized filter implementations are tested by an ensemble of filters with varying zero and pole positions.
Optimized filter coefficients are obtained by defining the filter objective function in L1 sense using RCGA.
Optimal value of fractional derivative constraints for optimized filter coefficients are determined by minimizing the objective function constructed using a sum of maximum passband ripple and maximum stopband ripple in frequency domain using CS algorithm.
Fluorescence was monitored at zoom 0.7 using optimized filter and mirror sets for the fluorophores in the respective scanning steps.
