Sentence examples for by the normalization factor from inspiring English sources

In the present work, among the five reference genes tested (Table 1), ubiquitin (UBQ) and cyclophilin (CYP) had the lowest expression stability mean values (M-value = 0.0257), i.e., these genes had more stable expression than other evaluated genes, and the data were normalized by the normalization factor calculated using these most stable reference genes.

The raw Cq value (i.e. non-normalized) for each candidate gene in each sample was divided by the normalization factor (NF). Subsequently, the normalized value for each candidate gene in the target was divided by the normalized value for the corresponding gene in the calibrator to generate relative expression of flavonoid biosynthetic pathway genes in virus-infected leaves.

After re-calculating the normalization factors using geNorm, the highest M value from the three best control genes was reduced to 0.68 and the raw expression values for the genes of interest in each cDNA were divided by the normalization factor for that cDNA to produce the normalized expression data.

Finally the FC of GOI expression was calculated with the ΔCTGOI multiplied by the normalization factor.

For normalization, the highest expression values for each gene were set to 1 and subsequently divided by the normalization factor generated by the GeNorm applet.

Normalized read counts were determined by dividing the raw number of reads aligned to each gene by the normalization factor calculated for that gene.

Subsequently, raw ddPCR values for HIV-1 usRNA were divided by the normalization factors to reach normalized data and reported as c/106 PBMCs for each patient sample.

Relative quantities were divided by the normalization factors which were the geometric means of the two reference genes quantities.

In this paper, we complete the normalization with hypothesis test, and search a normalization factor by iterating, then use the normalization factor to detect the DE genes.

geNorm software was used to establish the two most stable internal control genes (MRPL19 and PUM1) from a group of four endogenous controls (ACTIN, GAPDH, PUM1 and MRPL19), followed by the calculation of the normalization factor for each tissue sample (Table S2 in Additional file 1).

All the reactions were carried out in duplicate, and fold changes in gene expression were determined by using the formula 2-ΔΔ C t. geNorm software [ 32] was used to establish the two most stable internal control genes (MRPL19 and PUM1) from a group of four endogenous controls (ACTIN, GAPDH, PUM1 and MRPL19), followed by the calculation of the normalization factor for each tissue sample.